Genotyping of knockout (KO) mice

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Knockout (KO) mice are genetically modified (transgenic) mice in which one or more genes are turned off. Such animal models can be helpful tools in providing information of the biological function of a gene.

At the institute we use several mouse strains with different gene modifications. The genotypes of these mice are determined by PCR (polymerase chain reaction), a method used to amplify a specific region of a DNA strand. The PCR products are analysed by agarose gel electrophorersis to check for precence or not of DNA fragments of interest.

The figure shows results from genotyping of mice with an inducible cardiomyocyte-specific excision of the Atp2a2 (Serca2) gene (SERCA2 KO) (K. Andersson, G. Christensen). The upper band represents Cre-recombinase wich is expressed in the KO only , the lower band represents an internal control, thus samples with two bands are from SERCA2 KO mice, samples with one band are from control mice.

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